General description

Research area: Neuroscience

Tau-383 belongs to the neuronal microtubule-associated protein family and is found in the axons of the CNS (central nervous system). It is encoded by the MAPT (microtubule associated protein tau) gene on chromosome 17q21 and exists in the form of 6 isoforms produced by the alternative splicing of m-RNA transcripts of MAPT (microtubule associated protein tau).

Application

The product can be utilized for systemic identification of Phosphorylation sites in microtubule-associated protein tau by using checkpoint kinases Chk1 and Chk2 in vitro.

Biochem/physiol Actions

Microtubule-associated protein tau is mainly involved in promoting the assembly and stability of microtubules in axons. The N terminal domain of tau protein is responsible for many important functions including regulation of microtubule dynamics, and spacing between microtubules and cell compartments. While the proline rich domain of the tau protein plays a key role in neuronal signaling, functioning, and maintenance of the cytoskeleton. The interaction between tau and the microtubules plays a crucial role in regulating cell signaling, synaptic plasticity, and genomic stability. Thus, an impaired interaction between Tau and microtubules leads to the development of several neurodegenerative diseases.

Isoform of Tau, variant 0N4R, having 4 microtubule binding repeats (R) and no amino terminal inserts (N)

Reconstitution

Lyophilized from MES, pH 6.8, containing NaCl and EGTA. When reconstituted in water to a protein concentration of 1 mg/mL, the resulting buffer will have ~50 mM MES, pH 6.8, 100 mM NaCl, and 0.5 mM EGTA.