TB-Medium Base according to Loewenstein-Jensen (C15-1307-155)

Application

The selective Lowenstein Jensen Medium is used for isolation of Mycobacteria sp. from contaminated specimens. Egg supplement enhances Mycobacteria growth. Malachite green, penicillin* and nalidixic acid* inhibit Gram-negative and Gram-positive bacteria. (* included in the Gruft Supplement)

Preparation Note

Suspend 37,2 g in 600 ml of purified water containing 12 ml of glycerol (Cat. No. 49769). Heat with frequent agitation and boil for one minute. Sterilize at 121°C for 15 minutes. Cool to 45-50°C and, aseptically, add 1 L of homogenised sterile eggs and if required 4 vials of Gruft Mycobacterial Supplement (Cat. No. 51803). Dispense into sterile culture tubes and coagulate at 85°C for 45 minutes in slant position.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

sterility: non-sterile. Quality Level: 200. form: powder. shelf life: limited shelf life, expiry date on the label. composition: L-asparagine, 2.25 . g/L , magnesium citrate, 0.375 . g/L , magnesium sulfate, 0.15 . g/L , malachite green, 0.25 . g/L , monopotassium phosphate, 1.5 . g/L , potato flour, 18.75 . g/L . manufacturer/tradename: NutriSelect^®. Plus. technique(s): microbiological culture: suitable. application(s): clinical testing
environmental
food and beverages
life science and biopharma
veterinary, microbiology. suitability: mycobacteria. Pictograms: GHS07. Signal Word: Warning. Hazard Statements: H319. Precautionary Statements: P264 - P280 - P305 + P351 + P338 - P337 + P313. Hazard Classifications: Eye Irrit. 2. Storage Class Code: 11 - Combustible Solids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: Eyeshields, Gloves, type N95 (US).