TBST [10X]; Tris buffered saline with Tween-20; 100mM Tris.HCl, 1.5M NaCl, 0.5% Tween-20, pH 7.5, 1L

 A 10X Concentrated solution of Tris Buffered Saline with Tween® 20.    The 10X Concentration is 100mM Tris.HCl, 150mM NaCl, 0.5% Tween® 20 at pH7.5.   The 1X Concentration is 10mM Tris.HCl, 15mM NaCl, 0.05% Tween® 20 at pH7.5.   TBST is commonly used as a wash solution for Western blot membranes and microtiter plate wells in ELISA assays. The Tris Buffered Saline with Tween® 20 is an optimal formulation of pH stabilizers, salts and detergents designed to effectively remove excess material from membranes and microtiter plate wells without disrupting the antigen/antibody binding reaction.   By maintaining the proper buffering environment, unbound components can be washed away without suppressing antigen-antibody binding interactions, thereby reducing nonspecific background and increasing the specific signal.   Features  1X Concentration is 10mM Tris.HCl, 15mM NaCl, 0.05% Tween® 20 at pH7.5   Applications  Wash buffer for Western Blotting and ELISA