TE, 1X pH 8.0 (Tris EDTA) 100mL

TE is composed of buffering agent Tris, and chelating agent EDTA. EDTA prevents degradation of DNA and RNA by chelating magnesium (or other divalent metal ions). Together, this Tris-EDTA based solution solubilizes DNA or RNA, while protecting it from degradation. Filtered through a 0.22 µm membrane DNase, RNase, and Protease tested Applications The use of TE with slightly different pH values is based on early 80’s literature that DNases are supposed to be less active at pH 8.0 and RNases less active at pH 7.5. You can use pH 8.0 for both DNA and RNA. TE is useful for reconstituting nucleic acids, during DNA precipitation and other DNA applications, storing and diluting DNA and RNA, breaking protein-links in immunohistochemistry procedures, and unmasking antigens and epitopes in formalin-fixed and paraffin-embedded tissue sections.