T767021Component250 U1000 UStorageT767021ATelN Protelomerase25 μL100 μL-20°C. Avoid freeze/thaw cycleT767021B10×TelN Reaction Buffer250 μL1 mL-20°C. Avoid freeze/thaw cycle TelN Protelomerase, whose Chinese name is TelN Prokaryotic telomerase, is cloned from phage N15, which cuts dsDNA at the recognition site TelRL (56bp), The TelRL site consists of a palindromic sequence TelO in the middle and a palindromic sequence R3 and L3 at both ends of 14bp (Fig.1), and leaves a covalently closed end at the cleavage site, thus effectively converting circular DNA into linear DNA with hairpin ends (Fig.2).SourceE.coliEnzyme Activity DefinitionThe amount of enzyme required to completely cut 0.5 μg linearized pMiniT-TelN control plasmid (313 fmol TelN recognition site) for 30 min in a 50 μl system at 30°C was defined as 1 unit of activity (U).Protocol(1) Configure the mixture as shown in the following table: ComponentvolumedsDNAX μl10×TelN Reaction Buffer2 μlTelN Protelomerase1 μlNuclease-free WaterTo 20 μl(2) Shake the mixed system gently up and down, and centrifuge briefly.(3) Incubate at 30℃ for 30 min.(4) Incubation at 75℃ for 5 min terminated the reaction.Cautions(1) This product is for scientific research purposes only and shall not be used for other purposes (since 2022.05.31).(2) In some application scenarios, there are patent restrictions on the use of this enzyme.
Specifictaions: EnzymoPure™, BioReagent, for DNA synthesis, for DNA and RNA applications, PCR Reagent, Suitable for molecular biology, For In Vitro Transcription, 10 U/μL
- UPC:
- 51281625
- Condition:
- New
- Availability:
- 5-10 days
- Weight:
- 7.06 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- T767021-1000U
- Product Size:
- 1000U
