Terrific Broth II, Powder, 5kg

Terrific Broth II is a nutritionally rich medium for the growth of E. coli cells containing yeast extract and tryptone in elevated concentrations as well as sodium chloride and vitamins for a luxurious growth. Molecular Biology Certified Proprietary formulation that is shipped as a single powder Exhibits the same growth characteristics as the original Terrific Broth formula. Terrific Broth II is a rich nutrient media for propagation of Escherichia coli for purposes of strain maintenance, cloning, protein expression. It is certified for plasmid and other bacterial cultures in liquid medium. Higher cell densities are achieved using Terrific Broth in comparison to traditional LB Broth. Reagents are tested and chosen for their ability to promote optimum growth of E. coli when combined in Terrific Broth II formulations. Chosen reagents are combined in the accurate proportions according to the formula and thoroughly blended for a uniform distribution. A sample of the blended formulation was used to prepare liquid. The combinations were mixed to dissolve components and autoclaved at 121°C for 15 minutes.