Tetanus Toxin, Clostridium tetani - Calbiochem (C15-1306-559)

General description

Produced by the anaerobic bacillus Clostridium tetani. Tetanus toxin is one of the deadliest toxins known. Composed of two polypeptide chains joined by a single interchain disulfide bond. The heavy chain (100 kDa) participates in binding to the nerve terminals, internalization, retrograde axonal transport, and membrane translocation of the light chain (50 kDa) into the neuronal cytosol. The light chain inhibits neurotransmitter release after reduction of the disulfide bridge.

Produced by the anaerobic bacillus Clostridium tetani, Tetanus toxin is one of the deadliest toxins known. Composed of two polypeptide chains joined by a single interchain disulfide bond. The heavy chain (~100 kDa) participates in binding to the nerve terminals, internalization, retrograde axonal transport, and membrane translocation of the light chain (~50 kDa) into the neuronal cytosol. The light chain inhibits neurotransmitter release after reduction of the disulfide bridge.

Tetanus toxin is produced by Clostridium tetani. Composed of a 100 kDa heavy and a 50 kDa light chain joined by a single interchain disulfide bond.

Biochem/physiol Actions

Binds G_T1b ganglioside in a hemagglutination assay

Primary Target
Tetanus toxin is one of the deadliest toxins known

Product does not compete with ATP.

Warning

Toxicity: Highly Toxic (H)

Reconstitution

Following reconstitution, refrigerate (4°C). Stock solutions are stable for up to 1 month at 4°C.

Please refer to the vial label for lot-specific reconstitution volume.

Analysis Note

Single band by SDS-PAGE

Other Notes

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Montecucco, C., et al. 1994. FEBS Lett. 346, 92.
Wellhöner, H.H., et al. 1987. J. Biol. Chem. 262, 17374.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Quality Level: 100. form: lyophilized. manufacturer/tradename: Calbiochem^®. . availability: not available in Canada, . storage condition: OK to freeze. storage temp.: 2-8°C. Pictograms: GHS06,GHS08. Signal Word: Danger. Hazard Statements: H300 + H330 - H370. Precautionary Statements: P260 - P264 - P270 - P271 - P284 - P304 + P340 + P310. Hazard Classifications: Acute Tox. 1 Oral - Acute Tox. 2 Inhalation - STOT SE 1. Target Organs: Central nervous system. Storage Class Code: 6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials. WGK: WGK 3.