Cell Line Origin
Human erythroleukaemia
Cell Line Description
TF-1 have been derived from a patient with erythroleukaemia. They show complete growth dependency on GM-CSF or IL-3, and carry a homogeneous chromosomal abnormality (54,X). Constitutive expression of globin genes indicate commitment of TF-1 to the erythroid lineage, although glycophorin A or carbonyl anhydrase I are not expressed. On incubation with hemin or delta-aminolevulinic acid haemoglobin synthesis was induced, whereas TPA induced differentiation into macrophage-like cells. Erythroprotein also sustains short-term growth, but does not induce differentiation.
Application
TF1 cell line has been used-
- for tumorigenic studies
- to study the effect of granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-3 (IL-3) on proliferation and TNFR2 expression in TF1 cells
- in a cell-based proliferation assay to study the biological activities of anti-IL-6R Nanobody ALX-0061
- to study the induction of STAT5 phosphorylation by IL-3
DNA Profile
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 13
D13S317: 8,9
D16S539: 9,12
D5S818: 13
D7S820: 12
THO1: 7,9
TPOX: 8
vWA: 15,17
Culture Medium
RPMI 1640 + 2mM Glutamine + 1% Sodium Pyruvate (NaP) + 2-5ng/ml Human Granulocyte Macrophage Colony Stimulating Factor (Human GM-CSF) + 10% Foetal Bovine Serum (FBS). It is recommended to add fresh GM-CSF every 48 hours.
Subculture Routine
Maintain cultures between 2-9x100,000 cells/ml; 5% CO2; 37°C.
Other Notes
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biological source: human blood. growth mode: Suspension. karyotype: Carries homogeneous chromosomal abnormality (54,X). morphology: Not specified. products: Not specified. receptors: Interleukin 3 (IL-3) and Granulocyte Macrophage Colony Stimulating Factor (GM-CSF). technique(s): cell culture | mammalian: suitable. relevant disease(s): cancer. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 41106514
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 93022307-1VL