TH34 (C09-1074-860)

InformationTH34 is aHDACinhibitor that shows pronounced selectivity for HDACs 6, 8 and 10 over HDACs 1, 2 and 3. In a NanoBRET assay, TH34 strongly binds HDAC6, 8 and 10 with low-micromolar IC50 concentrations (HDAC6: 4.6 µM, HDAC8: 1.9 µM, HDAC10: 7.7 µM).TargetsHDAC8 ; HDAC6 ; HDAC10 1.9 μM; 4.6 μM; 7.7 μMIn vitroTH34 strongly binds to HDAC6, 8 and 10 with low-micromolar IC50 concentrations (HDAC6: 4.6 µM, HDAC8: 1.9 µM, HDAC10: 7.7 µM), and shows no substantial affinity to HDAC2 at concentrations up to 50 µM. Its treatment induces hyperacetylation of tubulin and SMC3. TH34 effectively and selectively eliminates high-grade neuroblastoma cells while sparing non-transformed human cells. In neuroblastoma cell lines as well as primary neuroblastoma cells, it markedly induces DNA damage, followed by differentiation and G2/M phase cell cycle arrest at later timepoints, eventually leading to cell death.Cell Research(from reference)Cell lines：SK-N-BE(2)-C cells Concentrations：10 μM Incubation Time：48 h. Specifications and Purity: 10mM in DMSO. Molecular Formula: C15H16N2O2. Molecular Weight: 256.3. PubChem CID: 134159676. Isomeric SMILES: CC1=C(C=C(C=C1)C(=O)NO)NCC2=CC=CC=C2.