THP 1 HUMAN MONOCYTIC LEUKAEMIA CELLS (C15-1311-336)

Cell Line Origin

Human monocytic leukaemia

Cell Line Description

Derived from the peripheral blood of a 1 year old male with acute monocytic leukaemia. THP-1 cells have Fc and C3b receptors and lack surface and cytoplasmic immunoglobulins. These cells also stain positive for α-napthhyl butyrate esterase, produce lysozymes and are phagocytic (both latex beads and sensitised erythrocytes). THP-1 cells can also restore the response of purified T lymphocytes to Concanavlin A, show increased CO₂ production on phagocytosis and can be differentiated into macrophage-like cells using for example DMSO.

Application

THP 1 cell line human has been used to study:

• bactericidal activity of moxifloxacin against Staphylococcus aureus
• the effect of lipopolysaccharide-mediated inflammatory response in murine versus human cell lines
• the effects of purine nucleosides on the in vitro growth of Cryptosporidium parvum
• the change in gene expression after infection with Mycobacterium tuberculosis
• the induction of cytokines in the cell line in presence of antigens obtained from Clostridium difficile

DNA Profile

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,13
D13S317: 13
D16S539: 11,12
D5S818: 11,12
D7S820: 10
THO1: 8,9.3
TPOX: 8,11
vWA: 16

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS / FCS).

Subculture Routine

If starting from a frozen ampoule the cryoprotectant should be removed. Add thawed cells to a conical based centrifuge tube, e.g. 15ml tube, slowly add 4 ml of culture medium to the tube. Take a sample of the cell suspension, e.g. 100μl, to count cells. Centrifuge the cell suspension at low speed i.e. 100 - 150 x g for a maximum of 5 minutes. Remove medium and resuspend the cell pellet at a density of 3 - 5 x 100,000 cells/ml in fresh medium containing 20% serum. Incubate flask at 37°C; 5 - 7% CO2. Check daily. Keep flask in a vertical position until the cells reach the exponential phase of growth. This can take up to 7 days. Once the culture is established the serum concentration can be reduced to 10%. To keep the cells in exponential growth, maintain cultures between 3-8x100,000 cells/ml. Requires 5% DMSO and 95% fetal bovine serum (FBS) as cryoprotectant. Growing orders are recommended due to difficulties that can be experienced during the initial start-up of this cell line. Replacements will be charged at full cost where claims cannot be substantiated.

Other Notes

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biological source: human blood. growth mode: Suspension. karyotype: Not specified. morphology: Monocyte. products: Not specified. receptors: Fc, C3b. technique(s): cell culture | mammalian: suitable. relevant disease(s): cancer. shipped in: dry ice. storage temp.: −. 196°C.