Cell Line Origin
Mouse Sp2/0 x Mouse BALB/c spleen
Cell Line Description
The antibody is cytotoxic for Leu-5 positive cells. The cells require NaP, oxaloacetic acid and bovine insulin in the growth medium.
Immunogen
Human lymphocytes (HLA phenotype A2, A28, B27, Bw4, Cw2)
Culture Medium
RPMI 1640 + 2mM glutamine + 1% Non Essential Amino Acids (NEAA) + 0.05mM 2-mercaptoethanol (2ME) + 10% Foetal Bovine Serum (FBS)
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.
Other Notes
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biological source: Mouse x Mouse Hybridoma. ImmunoDon: BALB/c mouse spleen. Myeloma: Sp2/0. Reactivity: Anti-Leu-5. technique(s): cell culture | mammalian: suitable. isotype: IgM. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 51313302
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 92100624-1VL