General description
Insoluble TMB substrate supplied as a single component system. TMB is the most sensitive chromogenic substrate for detection of horseradish peroxidase (HRP)-labeled probes. In the presence of HRP and hydrogen peroxide (H2O2), TMB is oxidized first to a blue, cation free radical; upon further reaction with HRP/H2O2, or addition of acid, the radical is converted to a yellow diimine terminal oxidation product. TMB is used routinely in ELISA procedures, whereby its soluble oxidation products are measured spectrophotometrically. For TMB to be worthwhile as a blotting reagent, enhancers such as dextran sulfate or other sugar polymers are usually added.This TMB reagent is a safe, single component system that has been developed for use in blotting procedures by a proprietary methodology that eliminates the use of sugar polymers. The reagent produces a stable, insoluble aquamarine precipitate at the reaction site with little or no background. It can significantly increase the detection limits of assays on a variety of membranes. Ideal for many immunoblotting procedures and can significantly increase detection limits of assays on a variety of membranes
Peroxidase substrate that produces a stable, insoluble, aquamarine blue precipitate at reaction site with little or no background.
Warning
Toxicity: Carcinogenic / Teratogenic (D)
Physical form
1.19 mM H₂O₂ in a proprietary solvent, 1.13 mM TMB, pH 4.9 ± 0.03.
Reconstitution
Discard if the solution turns blue or turbid. The reagent is stable at room temperature; refrigeration is recommended for long-term storage. Warm to assay temperature before use.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
- UPC:
- 51452501
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 613548-100ML