This following method is suggested as a guideline for the use for Western Blot with nitrocellulose or PVDF membranes. The TMB Substrate in 100 mL bottle TMBM-101 and the TMB Precipitating Agent in 10 mL bottle TMBM-102 are used together. Mix 9 ml of the TMB Substrate and 1.0 ml of the TMB Precipitating Agent and mix thoroughly. The substrate is now ready to use. Be certain to use within 24 hours. After antibody incubations and wash, aspirate the wash solution and add approximately 5 ml of freshly prepared substrate solution. Incubate until color develops (usually 2 to 20 min). Wash the membrane with 2 changes of deionized water for 5 min each.
- UPC:
- 12350000
- Condition:
- New
- Availability:
- 3 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- TMBM-101
