TNE Buffer 500mL

TNE Buffer is often used as a base material to generate lysis buffers. Filtered through a 0.22 µm membrane DNase, RNase, and Protease tested Applications More specifically, TNE Buffer is useful for: isolation of proteins from yeast cells as a lysis buffer, resuspension of pelleted virus or reporter virus particles and the flurometric determination of DNA content. Recommendation Without correcting the pH, concentrated virus can be used in non-denaturing SDS/PAGE-Western Blot analysis. If the pH is corrected to inhibit triggering of the viral fusion machinery (for West Nile Virus, this corresponds to pH 7.4), the concentrated virus will remain infectious.