Anti-TNF p55 Receptor (sTNFRp55) antibody has been tested for use in ELISA, radioimmunoassays, immunoblotting, flow cytometry and immunoprecipitation. Reactivity in other immunoassays is unknown. This product has been assayed for the ability to immunoprecipitate antigen and a dilution range of 1:400 to 1:800 is suggested. For immunoprecipitation, pre-clearing with a non-specific rabbit IgG is helpful to reduce background. This product has been assayed by immunoblot and a working dilution range of 1:200 to 1:400 is suggested for this application. This product has been assayed by ELISA against soluble (extracellular) sTNFRp55 at a dilution range of 1:2,000 to 1:8,000. For use in ELISA formats, this antibody is best used as the second antibody in combination with a monoclonal antibody as a capture antibody. This product has been assayed by radioimmunoassay against antigen. A dilution of 1:8,000 is suggested for this immunoassay.
- UPC:
- 41106200
- Condition:
- New
- Availability:
- 3 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 209-401-308
