Cell Line Origin
Rat Wistar-Furth oligodendroglioma
Cell Line Description
Ethyl nitrosourea induced tumour of the spinal cord and roots from a Wistar-Furth rat. In cell culture the cells grow to a high density and form a sheet of cells with many processes. TR33B is the parent line of 33B (Sigma Catalogue number. 85081901).
Application
Tumourigenicity studies
Culture Medium
DMEM + 2mM Glutamine + 10% Tryptose Phosphate Broth + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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biological source: rat brain. growth mode: Adherent. karyotype: Not specified. morphology: Glial. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. relevant disease(s): cancer. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 51142512
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 85040102-1VL