General description
Recombinant, human TRAIL (amino acids 95-281) with an amino-terminal 6X histidine tag and expressed in NSO cells. A type II transmembrane protein with a C-terminal extracellular domain that exhibits homology to other members of this tumor necrosis factor (TNF) family. Displays potent anti-tumor activity against selected targets. Induces apoptosis in a number of transformed cell lines but not in normal tissues. Recombinant bioactive TRAIL is a non-disulfide-linked homotrimer but may contain a small amount (up to 10%) of disulfide-linked homodimers.
Recombinant, human TRAIL expressed in NSO cells a type II transmembrane protein with a C-terminal extracellular domain, which exhibits homology to other TNF family members. Displays potent antitumor activity against selected targets. Induces apoptosis in a number of transformed cell lines but not in normal tissues.
Biochem/physiol Actions
ED₅₀ = 4-12 ng/ml as measured by its ability to induce cytotoxicity in mouse L-929 cell line in the presence of metabolic inhibitors actinomycin D
Warning
Toxicity: Standard Handling (A)
Physical form
Lyophilized from sterile-filtered PBS, 50 µg BSA/µg TRAIL, pH 7.4.
Reconstitution
Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C or -70°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or up to 3 months at -20°C or -70°C. Avoid freeze/thaw cycles of solutions.
Reconstitute to ≥20 µg/ml in sterile PBS, 0.1% BSA.
Other Notes
Griffith, T.S., et al. 1999. J. Exp. Med.189, 1343.
Mariani, S.M., et al. 1997. J. Cell Biol.137, 221.
Pan, G., et al. 1997. Science277, 815.
Pan, G., et al. 1997. Science276, 111.
Sheridan, J.P., et al. 1997. Science277, 818.
Pitti, R.M., et al. 1996. J. Biol. Chem.271, 12687.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
recombinant: expressed in NSO cells. Quality Level: 100. Assay: ≥. 97% (SDS-PAGE). form: lyophilized. manufacturer/tradename: Calbiochem®. . storage condition: OK to freeze. impurities: ≤. 1 . EU/μ. g Endotoxin (EU/μ. g TRAIL). shipped in: ambient. storage temp.: −. 70°C. Storage Class Code: 11 - Combustible Solids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.- UPC:
- 12352206
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 616376-10UG