General description
A recombinant reverse transcriptase for the robust transcription of RNA fragments up to 14 kb used in two-step RT-PCR on thermal cyclers and real-time PCR instruments.
In retroviruses like the human immunodeficiency virus type 1 (HIV-1), reverse transcriptase (RT) is the core enzyme. HIV-1 RT is made of two subunits of 66 kDa and 51 kDa (p66 and p5l). A human endogenous retrovirus of the HERV-K family codes for reverse transcriptase (RT) enzyme.
Application
Transcriptor Reverse Transcriptase is designed to transcribe RNA (mRNA, total RNA, viral RNA, and in vitro-transcribed RNA) from a variety of sources, using conventional thermal cyclers and real-time PCR instruments (e.g., the LightCycler® Instruments) for the following applications:
- Synthesis of first-strand cDNA for use in subsequent amplification reactions
- RT-PCR of GC-rich RNA templates
- Cy3, Cy5, DIG, biotin, and aminoallyl labeling during cDNA synthesis
- Retrieving and cloning the 5′ and 3′ termini of mRNA by RACE
- Generation of cDNA libraries with large inserts
- Dideoxy DNA sequencing
- RNA sequencing
- 3′-end labeling of DNA fragments
- Generation of single-stranded probes for genomic footprints
- In the reverse transcription of RNA from human Papillomavirus E6, cortical and striatal tissues, muscle biopsies of Becker muscular dystrophy samples and miRNA stem-loop from oocytes and human cerebral microvascular endothelial cells (hCMVECs)
Biochem/physiol Actions
Human immunodeficiency virus type 1 reverse transcriptase (HIV-1 RT) is essential for the catalytic conversion of single-stranded viral RNA into the double-stranded linear DNA that is integrated into host cell chromosomes.
Features and Benefits
- Achieve high sensitivity in two-step RT-PCR.
- Obtain more full-length transcripts - up to 14 kb.
- Reverse transcribe difficult templates.
- Efficiently label cDNA.
Components
- Transcriptor Reverse Transcriptase, in storage buffer
- Transcriptor RT buffer, 5x concentrated
Quality
Each lot of Transcriptor Reverse Transcriptase is routinely function tested in RT-PCR:
- with a conventional thermal cycler to detect a 10-kb fragment from the human dystrophin gene, starting from human skeletal muscle total RNA
- with the LightCycler® Instrument to detect 5 x 102 to 5 x 106 copies of in vitro-transcribed human PBGD RNA. The results are defined in fixed crossing points and fixed fluorescence intensity.
Preparation Note
Volume activity: 20 U/μl.
Transcription of long, rare, or difficult RNA targets
Transcriptor Reverse Transcriptase is recommended for RT-PCR of:
- long targets, because it can transcribe up to 14 kb RNA templates
- rare targets, because it has high sensitivity
- GC-rich targets, because it can operate at high temperatures (up to +65oC) to eliminate problems associated with extensive secondary structure
Labeling for many applications
Transcriptor Reverse Transcriptase is also recommended for preparing labeled cDNA, since it accepts a wide variety of modified nucleotides (including Cy3-, Cy5-, DIG-, Biotin-, or aminoallyl-labeled dNTPs).
Reaction requirements
Transcriptor accepts ssRNA and ssDNA templates and requires a primer for transcription.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Legal Information
LightCycler is a registered trademark of Roche
usage: sufficient for 25 . reactions, sufficient for 50 . reactions, sufficient for 500 . reactions. Quality Level: 100. feature: dNTPs included: no, hotstart: no. manufacturer/tradename: Roche. packaging: pkg of 200 reactions (03531287001), pkg of 25 reactions (03531317001), pkg of 50 reactions (03531295001). technique(s): RT-PCR: suitable, RT-qPCR: suitable. detection method: probe-based. Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): does not flash. Flash Point(C): does not flash.- UPC:
- 12352204
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 3531287001