General description
TransPlex®, a Whole Transcriptome Amplification (WTA) method, allows for representative amplification of nanogram quantities of total RNA in less than 4 hours without 3′-bias. Microgram quantities of amplification product generated from tissue, cultured cells, formalin-fixed samples, or serum are suitable for downstream applications such as qPCR and microarray analyses.
Application
TransPlex® Whole Transcriptome Amplification Kit has been used to synthesize double-stranded cDNA. It has also been used in the amplification of cDNA.
Suitable for use with downstream applications including:
- qPCR
- microarray analysis
- cloning
Features and Benefits
- Amplification of total RNA in less than 4 hours with less than 30 minutes of "hands-on" time
- Only 5 ng of starting material required to produce a highly representative library from total RNA
- Microgram quantities of amplification product generated from intact RNA from tissue, cultured cells, serum, or degraded RNA from formalin-fixed paraffin-embedded samples
Principle
The WTA process involves two steps. In the first step, sample RNA is reverse transcribed with non-self-complementary primers composed of a quasi-random 3′ end and a universal 5′ end. As polymerization proceeds, displaced single strands serve as new templates for primer annealing and extension. The resultant OmniPlex cDNA library, comprised of random, overlapping 100 - 1000 base fragments flanked by universal end sequence, is then amplified by PCR with the universal primer to produce WTA product.
Legal Information
TransPlex is a registered trademark of Rubicon Genomics, Inc.
Shipping Information:
Dry Ice Surcharge & Ice Pack Shipments: $40
More Information: https://cenmed.com/shipping-returns
- UPC:
- 41106312
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- WTA1-50RXN
- Temperature Control Device:
- Yes
