Tributyrin HiVegT Agar Base without Tributyrin is for detect (C08-0443-925)

Tributyrin HiVeg Agar Base is specially developed from HiVeg peptone to avoid BSE/ TSE risks associated with animal origin Peptic digest of animal tissue. This medium is the modification of Tributyrin Agar Base, which was originally formulated by Anderson (1) for the detection and enumeration of lipolytic microorganisms such as Staphylococci (2), Clostridia (3), marine Flavobacteria and Pseudomonas (4) and moulds in foodstuffs and other materials. HiVeg peptone and yeast extract provide necessary nutrients for growth of the microorganisms. Tributyrin added in the medium on hydrolysis by esterase enzyme produces butyric acid which is water soluble therefore clearance is seen around the colony which produce the enzyme. Lipases hydrolysing low molecular weight or short chain triglycerides are refered as esterases. This is an invitro method followed to isolate lipolytic organisms and is used for screening lipase/ esterase producers. The medium should be properly mixed so that tributyrin substrate is distributed uniformly in the prepared plate. Directions: Suspend 23 grams in 990 ml distilled water. Add 10 ml of Tributyrin. Mix and heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Shake the flask and pour plate so as to maintain uniform turbidity.