Tris(2-carboxyethyl)-phosphine hydrochloride (TCEP) is a strong reducing agent that is stable in an aqueous solution. It readily and stoichiometrically reduces disulfides with high specificity. TCEP exhibits reducing activity at pH 1.5 to pH 9.0 and reduces dehydroascorbic acid (DHA) at pH 4.Application:Tris(2-carboxyethyl)phosphine hydrochloride has been used:as a component of IRE1 dialysis buffer, RNA cleavage buffer, and in in vitro cleavage assay of in vitro transcribed RNA in reducing sulfhydryl groups to obtain total thiol fractionto reduce protein samplesWater soluble reagent, used for selective reduction of disulfides. More stable than DTT and useful in mass spectrometry applications.
Specification: BioUltra, ≥98%(NMR)
MDL Number: MFCD00145469
Molecular Formula: C9H15O6P·HCl
Molecular Weight: 286.65
PubChem CID: 2734570
Isomeric SMILES: C(CP(CCC(=O)O)CCC(=O)O)C(=O)O.Cl
Beilstein Registry Number: 3724376
- UPC:
- 12352200
- Condition:
- New
- Availability:
- 4-8 weeks
- Weight:
- 0.07 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- T755741-1g
- CAS:
- 51805-45-9
- Product Size:
- 1g
- Hazard Statement Codes:
- H314
