Application
Tris-EDTA buffer solution has been used as a component in Tris-EDTA (TE) buffer to resuspend DNA pellets for total DNA extraction from herbage samples and single-stranded carrier DNA extraction from salmon sperm.
Biochem/physiol Actions
Ethylenediaminetetraacetic acid (EDTA) and its salts are used in a variety of medical and pharmaceutical applications. It is a calcium chelator that also acts as an anticoagulant and a detoxicant in the case of heavy metal poisoning. EDTA is frequently used in analytical chemistry for complexometric titrations and several other applications. It is used as a food additive to bind metal ions due to its low toxicity.
Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Tris-EDTA (TE) buffer solution, pH 8.0 may also be used as a washing buffer.
Features and Benefits
- DNases, RNases, phosphatases, and protease-free
- Insoluble matter, passes filter test
grade: for molecular biology. Quality Level: 200. product line: BioUltra. form: liquid. impurities: DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected. pH: 8.0±0.2 (25 . °C). cation traces: Al: ≤. 1 . mg/kg, Ba: ≤. 1 . mg/kg, Bi: ≤. 1 . mg/kg, Ca: ≤. 5 . mg/kg, Cd: ≤. 1 . mg/kg, Co: ≤. 1 . mg/kg, Cr: ≤. 1 . mg/kg, Cu: ≤. 1 . mg/kg, Fe: ≤. 1 . mg/kg, K: ≤. 20 . mg/kg, Li: ≤. 1 . mg/kg, Mg: ≤. 1 . mg/kg, Mn: ≤. 1 . mg/kg, Mo: ≤. 1 . mg/kg, Ni: ≤. 1 . mg/kg, Pb: ≤. 1 . mg/kg, Sr: ≤. 1 . mg/kg, Zn: ≤. 1 . mg/kg. λ: neat. UV absorption: λ. : 260 . nm Amax: 0.04, λ. : 280 . nm Amax: 0.01. web metadata keyword.default: T10E1, pH 8.0. TE buffer, pH 8.0. BioUltra. DNAse-free. RNAase-free. protease-free. phospatase-free. DNA extraction. RNA extraction. nucleic acid preperation. DNA washing. DNA washing. DNA storage. RNA storage. DNA suspension. RNA suspension. biology grade buffer. PCR buffer. DNA sequencing. ligation. SMILES string: NC(CO)(CO)CO.OC(: O)CN(CCN(CC(O): O)CC(O): O)CC(O): O. InChI: 1S/C10H16N2O8.C4H11NO3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20. 5-4(1-6,2-7)3-8/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20). 6-8H,1-3,5H2. InChI key: VLEIUWBSEKKKFX-UHFFFAOYSA-N. Storage Class Code: 12 - Non Combustible Liquids. WGK: nwg. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: Eyeshields, Gloves.
- UPC:
- 12161700
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 93283-100ML
