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Triticum vulgaris lectin (WGA)

Catalog No.
C007B-573071
Mfr. No.
T140107-5mg
Mfr. Name
Aladdin Scientific
Qty/UOM
1
UOM
EA
Price: $789.20
List Price: $876.89

Triticum vulgaris lectin or Weat germ agglutinin (WGA) is isolated from Triticum vulgaris (Weat germ) and purified by affinity chromatography. The lectin has two subunits and a molecular weight of 36 kDa.

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Triticum vulgaris lectin or Weat germ agglutinin (WGA) is isolated from Triticum vulgaris (Weat germ) and purified by affinity chromatography. The lectin has two subunits and a molecular weight of 36 kDa. WGA selectively binds to N-Acetyl glucosamine (GlcNAc) and to N-acetylneuraminic acid (sialic acid) residues of glycoproteins and glycolipids. The WGA lectin agglutinates erythrocytes and most types of malignant cells. It agglutinates rabbit erythrocytes at < 0.1 μg/ml following trypsin treatment of the cells. Adding 300 mM N-Acetyle-D-Glucosamine gives an inhibition with a titer that is at least 8-fold lower than the control. Wheat germ agglutinin inhibits the C5a receptor interaction, which has implications in studies of receptor micro-heterogeneity and ligand binding sites. WGA together with Con A is the lectin most widely used as an analytical and preparative agent when studying glycoproteins and cell surface proteins. The immobilized lectin can be used for affinity chromatography of cells and sub-cellular particles. WGA is supplied as a white to pale yellow lyophilized powder from a buffer containing 10 mM CH3COONH4. No preservatives are added. The purity of WGA is determined by SDS- electrophoresis which generates three bands, corresponding to the three isolectins of WGA. Protein content is verified in spectrophotmetry.● Ultrapure quality ● Sugar specificity: N-acetylglucosamine and N-acetylneuraminic acid residues ● Agglutinates erythrocytes and most types of malignant cells ● Agglutinates rabbit erythrocytes at ≤0.1 μg/ml after treating the cells with trypsin ● Not blood group specificStudies of glycoproteins and glycolipids; Purification of membrane proteins; Affinity chromatography of cells and sub-cellular particles; Agglutination studies

Related Documents: https://ald-pub-files.oss-cn-shanghai.aliyuncs.com/aladdinsci/pdp/sds/1/T140107-SCI_482b69ff59e12e866c378abab4b9b31a.pdf

UPC:
51461515
Condition:
New
Availability:
4-8 weeks
Weight:
1.06 Ounces
HazmatClass:
No
WeightUOM:
LB
MPN:
T140107-5mg
CAS:
96508-08-6
Product Size:
5mg

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