General description
Triton™ X-114 is a nonionic detergent. It is a good detergent for solubilizing membranes having reversible phase-separation property useful for the separation of intrinsic membrane proteins. Solution of Triton™ X-114 is homogeneous at 0°C but phase separation into aqueous phase and a detergent phase occurs above 20°C. Phase separation of proteins in solutions of Triton™ X-114 has been investigated. This octylphenol ethoxylate is compatible with anionic, cationic, and other nonionic surfactants and chemically stable in most acidic & alkaline solutions.
Application
Triton™ X-114 has been used:
- In phase separation of integral membrane proteins
- In the purification of bacterial inclusion bodies
- To study the clouding phenomenon in promethazine hydrochloride, an amphiphilic phenothiazine drug
- To partition conditioned media into insoluble and soluble phases before Western blotting
- For solubilization of phosphatidylinositol 4-phosphate 5-kinase from bovine brain
Legal Information
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
- UPC:
- 51111869
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 93422-1L
