Triton™ X-100 (C09-1050-748)

General descriptionTriton™ X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfectionApplicationTriton™ X-100 has been used:• In immunohistochemistry for staining the Flat-mount retinas• Along with ice-cold PBS (phosphate buffered saline) in suspension of cells for cell DNA analysis and Annexin V assay• To permeabilise cells during Immunofluorescent microscopic studies• As a positive control in LDH assay to determine the cell membrane integrity• For estimating the lipase activity in postheparin plasma by using modified Belfrage and Vaughan radioenzymatic procedure• For the preparation of outer membrane protein exctract• As a component of extraction buffer along with tris-HCl, NaCl, CaCl2, ZnCl2, Brij 35 for homogenization of mice lung cells• In the treatment of tissue sections for Immunofluorescence labeling. Specification: Biochemical grade MDL Number: MFCD00128254 Molecular Formula: C8H17C6H4(OCH2CH2)nOH EC Number: 232-658-1 PubChem CID: 5590 Isomeric SMILES: CC(C)(C)CC(C)(C)C1=CC=C(C=C1)OCCO Beilstein Registry Number: 2315025 Related Documents: https://ald-pub-files.oss-cn-shanghai.aliyuncs.com/aladdinsci/pdp/sds/1/T109026-SCI_5bd8fd4edddb42d8c712f1cb87a0d37d.pdf