General description
Trizma® hydrochloride solution is a useful biological buffer.
Application
Trizma® hydrochloride solution may be used in the following studies:
- As buffer for the 2-D electrophoresis of rat fibroblast cell.
- As buffer for the rapid isolation of high molecular weight plant DNA (50,000 base pairs or more in length).
- Selective immunoprecipitation of biotin-labeled DNA with antibiotin IgG and Staphylococcus sp.
The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per degree C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Other Notes
Prepared with pH-adjusted Biotechnology Performance Certified Trizma Base in 18 megohm water and 0.2 μm filtered.
Legal Information
Trizma is a registered trademark of Sigma-Aldrich Co. LLC
- UPC:
- 51102855
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- T3038-1L
