TrueBlot® Protein A magnetic beads can be used for immunoprecipitation (IP) and Co-IP experiments as well as for antibody purification. For IP, target specific antibody is incubated with cell lysate. Protein A magnetic beads are then incubated with antigen-antibody complex at room temperature, washed and then eluted using elution buffer. The samples are then resolved by SDS-PAGE and analyzed by Western blotting. For antibody purification, Protein A magnetic beads are incubated with the antibody solution and then separated by magnets. After the unbound particulates are washed from the beads, the bound antibodies are eluted from the beads using the elution buffer. The beads are then magnetically separated from the eluted solution, which is removed manually.
- UPC:
- 41122409
- Condition:
- New
- Availability:
- 3 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- PA00-18-5
