General description
Trypan Blue is a tetrasulfonated anionic, hydrophilic azo dye derived from toluidine. The non-permeable cell membrane dye is a vital dye that selectively stains dead or non-viable cells, that pass through damaged but not through intact cell membranes.
Application
Trypan blue applications include:
- it is a routinely used for viability testing for diverse cell types including frozen sperm and aortic muscle cells exposed to antifungal agents.
- employed as a collagen stain in a Van Gieson procedure
- To stain amyloid, Trypan blue is considered as a blue alternative to Congo red in Puchtler-Bennhold stains.
- used as a fluorescent tracer of cell populations in embryology
- used as a tumor promoter modulating permeability of lysosomal membranes
Biochem/physiol Actions
Trypan Blue is excluded by most living cells, but can be taken into phagocytes and certain other cells.
Physical form
0.4% in 0.81% sodium chloride; 0.06% potassium phosphate, dibasic
- UPC:
- 41141711
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 93595-250ML