General description
Egg white trypsin inhibitor is an inhibitor for trypsin, plasmin and plasma kallikrein activity in serum-free cell-culture media. It does not inhibit metallo-, cysteine, and aspartic proteases or tissue kallikrein (serine protease).
Application
Chicken egg Trypsin Inhibitor is used for the termination of tissue disaggregation and for subcultivation procedures.
Trypsin Inhibitor has been used:
- as a component of lysis buffer to treat Tsc2fl/fl (control) and Tsc2fl/flLyz2-Cre (knockout [KO]) bone marrow-derived macrophages (BMDMs) before immunoblotting
- to inactivate trypsin post detachment of the stem cells in ventricular zone cell culture
- to neutralize trypsin post dissociation and trypsinization of brain tissues
- termination of tissue disaggregation and for subcultivation procedures
Preparation Note
Working concentration: 0
Working solution: Solvent is recommended in distilled water.
Stock solution: 1 mg/ml
Storage conditions (working solution): -15 to -25 °C
Stable for at least 6 months in distilled water or 1 mM HCI when stored frozen in aliquots. Avoid repeated freeze-thaw cycles.
Reconstitution
Both are soluble in water. Recommended stock solution: 1 mg/ml. Store frozen in aliquots at -15 to -25 °C. Stable for at least 6 months.
Storage and Stability
Store at 2 to 8 °C. (Store dry!)
Other Notes
For life science research only. Not for use in diagnostic procedures.
- UPC:
- 12352200
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 10109878001
