TRYPSIN SEQUENCING GRADE

General description

Trypsin Sequencing Grade is isolated from bovine pancreas as a highly purified and specific protease. Trypsin Sequencing Grade is a serine endopeptidase. It specifically cleaves peptide bonds at the carboxylic side of the basic amino acids Arg and Lys. Amide and ester bonds of Arg and Lys are also cleaved.

Specificity

The specificity of Trypsin Sequencing Grade is verified with the oxidized B-chain of insulin (insulin B_ox) as substrate. High concentrations of Trypsin Sequencing Grade (1 part by weight enzyme with 18 parts by weight insulin B_ox) are incubated for 18 hours to detect traces of chymotrypsin impurities.

Specificity (HPLC, with Insulin B_ox):
Cleavage after 1 hour: ≥90%;
Unspecific cleavage products after 18 hours: ≤10%

Application

Trypsin Sequencing Grade is used to to digest proteins in solution, in gels or on blotting membranes.
The enzyme is used for protein-structure elucidation, tryptic mapping, fingerprinting, sequence analysis, and translocation studies. Trypsin Sequencing Grade generates glycopeptides from purified glycoproteins and is suited for the digestion of proteins in polyacrylamide gels.

Quality

Purity: Free of impurities that may interfere with the separation of peptides in reversed-phase HPLC.

Preparation Note

Stabilizers: Trypsin is stable in 4 M.
Working concentration: 1/100 to 1/20 of the protein by weight (in solution); 1-5 μg/100 μl (for in-gel digest)
Storage conditions (working solution): A solution in 0.01% trifluoroacetic acid (TFA), (v/v) or 1 mM HCl may be used for one week at maximum, if stored at 2 to 8 °C. By incubation of proteins in solution at neutral to slightly basic pH-values partial autolysis might occur. For this application, Roche recommends Trypsin, Modified, Sequencing Grade.

Reconstitution

Reconstitution in acid is necessary for stability of solution: 0.01% TFA (v/v), 1 mM HCl or 0.1% acetic acid are recommended.

Other Notes

For life science research only. Not for use in diagnostic procedures.