General description
High efficiency, resin-based TSKgel affinity columns (10 μm particles) separate or purify many enzymes and other proteins.
Coupling of m-aminophenyl boronate to TSKgel 5PW polymeric support results in a ligand capable of forming a tetrahedral boronate anion under alkaline pH conditions. This anionic structure can bind with 1,2 cis-diol groups such as those found in carbohydrates, carbohydrate containing compounds and catecholamines. Interaction between the boronate anion and the 1,2 cis-diol groups is enhanced in the presence of Mg2+ ions and is inhibited by amine-containing buffers. Adsorption onto the TSKgel Boronate-5PW matrix takes place in basic buffers such as HEPES and morpholine while desorption takes place in carbohydrate or amine-containing mobile phase like sorbitol or Tris.
Applications include: nucleic acids, catecholamines, nucleosides and nucleotides.
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Legal Information
TSKgel is a registered trademark of Tosoh Corporation
material: PEEK . product line: TSKgel®. . analyte chemical class(es): proteins. manufacturer/tradename: Tosoh Boronate-5PW, 13066. parameter: ≤. 20% organic solvent, ≤. 3 M salt concentration, 1.2 mL/min flow rate, 30 bar max. pressure, ≥ 0 °C temperature. technique(s): HPLC: suitable. L × I.D.: 7.5 . cm × 7.5 . mm . matrix: polymer particle platform. matrix active group: m-aminophenyl boronate phase. particle size: 10 . μ. m. pore size: 1000 . Ã…. operating pH: 2-9 . capacity: 40 . μ. mol/mL adsorption capacity (sorbitol). compatibility: mode of use affinity HPLC. separation technique: affinity. storage temp.: 2-8°C. Storage Class Code: 10 - Combustible liquids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable.- UPC:
- 42321905
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 813066