General description
High efficiency, resin-based TSKgel affinity columns (10 μm particles) separate or purify many enzymes and other proteins.
Coupling of m-aminophenyl boronate to TSKgel 5PW polymeric support results in a ligand capable of forming a tetrahedral boronate anion under alkaline pH conditions. This anionic structure can bind with 1,2 cis-diol groups such as those found in carbohydrates, carbohydrate containing compounds and catecholamines. Interaction between the boronate anion and the 1,2 cis-diol groups is enhanced in the presence of Mg2+ ions and is inhibited by amine-containing buffers. Adsorption onto the TSKgel Boronate-5PW matrix takes place in basic buffers such as HEPES and morpholine while desorption takes place in carbohydrate or amine-containing mobile phase like sorbitol or Tris.
Applications include: nucleic acids, catecholamines, nucleosides and nucleotides.
Recommended products
Discover LiChropur reagents ideal for HPLC or LC-MS analysis
Legal Information
TSKgel is a registered trademark of Tosoh Corporation
material: glass . product line: TSKgel®. . analyte chemical class(es): proteins. manufacturer/tradename: Tosoh 14449. parameter: ≤. 20% organic solvent, ≤. 3 M salt concentration, >. 0 °C temperature, 1.2 mL/min flow rate, 30 bar max. pressure. technique(s): HPLC: suitable. L × I.D.: 5 . cm × 5 . mm . matrix: hydroxylated methacrylic polymer. matrix active group: m-aminophenyl boronate phase. particle size: 10 . μ. m. operating pH: 2-9 . capacity: 40 . μ. mol/mL, resin adsorption capacity (sorbitol). separation technique: affinity. storage temp.: 2-8°C.- UPC:
- 42321905
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 814449
