TSKGEL PHENYL-5PW RP 13UM 15CMX21.5MM (C15-1309-650)

General description

TSKgel Phenyl-5PW RP offers similiar selectivity to its hydrophobic interation counterpart TSKgel Phenyl-5PW, although a higher degree of cross-linking allows for increased stability in aqueous/organic mobile phase gradients. The highly cross-linked polymethacrylate base material provides an advantage over silica when high pH buffer systems are needed. Additionally, TSKgel Phenyl-5PW RP can withstand rigorous cleaning protocols using either acid or base. The 1000Ã… pore size accommodates globular protein samples up to 1,000,000 Da.

Polymer-based particles for reversed-phase (RP) HPLC generally have two benefits over silica-based particles: They are more tolerant of high and low pH operating conditions and they do not have the silanol interactions that cause peak tailing of some basic analytes. Also, some silicas have an intrinsic metal content that may cause chelation problems with sensitive biomolecules, leading to poor recovery. Polymeric TSKgel columns come in C18 and phenyl functional groups and a range of particle porosities. Fast kinetics and quantitative protein recovery at submicrogram loading are characteristic of nonporous resin-based TSKgel Octadecyl-NPR columns.

• Octadecyl-2PW particles have 125Ã… pores
• Octadecyl-4PW particles have 500Ã… pores
• Phenyl-5PW RP particles have 1000Ã… pores
• Octadecyl-NPR particles are nonporous

Physical form

Shipped in 30% methanol - 70% water.

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Legal Information

TSKgel is a registered trademark of Tosoh Corporation

material: stainless steel hardware. product line: TSKgel^®. . manufacturer/tradename: Tosoh Phenyl-5PW, 16260. extent of labeling: 1 . meq/mL ligand density (estimate). parameter: no limitation salt concentration, 0-100% organic solvent, 30 bar max. pressure, 4-60 °C temperature, 8 mL/min flow rate. technique(s): HPLC: suitable. L × I.D.: 15 . cm × 21.5 . mm . matrix: polymer particle platform. matrix active group: phenyl phase. particle size: 13 . μ. m. pore size: 1000 . Ã…. operating pH: 2-12. separation technique: reversed phase.