General description

Genotype: F^–ompT hsdS_B (r_B^– m_B^–) gal dcm lacY1(DE3) pLysS (Cam^R)

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Tuner strains are lacZY deletion mutants of BL21 and enable adjustable levels of protein expression throughout all cells in a culture. The lac permease (lacY) mutation allows uniform entry of IPTG into all cells in the population, which produces a concentration-dependent, homogeneous level of induction. By adjusting the concentration of IPTG, expression can be regulated from very low levels up to the robust, fully induced levels commonly associated with pET vectors. Lower-level expression may enhance the solubility and activity of difficult target proteins.

DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.

pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability.

Tuner host strains are lacZY deletion mutants of BL21, which enable adjustable levels of protein expression throughout all cells in a culture. T7 lysozyme expression suppresses basal T7 expression.

Components

0.4 ml1 mlComponent

•2 × 0.2 ml5 × 0.2 mlTuner(DE3)pLysS Competent Cells

•2 × 2 ml4 × 2 mlSOC Medium

•2 ng2 ngTest Plasmid

Warning

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

biological source: Escherichia coli. Quality Level: 100. manufacturer/tradename: Novagen^®.. storage condition: OK to freeze, avoid repeated freeze/thaw cycles. growth mode: adherent or suspension. morphology: rod shaped. technique(s): microbiological culture: suitable. cell transformation: transformation efficiency: >. 2Ã—10⁶ . cfu/μ. g. shipped in: dry ice. storage temp.: −. 70Â°C. Storage Class Code: 10 - Combustible liquids. WGK: WGK 2.