Cell Line Origin
Human Osteosarcoma
Cell Line Description
Cell line derived in 1964 from a moderately differentiated sarcoma of the tibia of a 15 year old girl.
Application
U-2 OS has been used to study:
- the importance of cyclin D1 for the activity of lithocholic acid hydroxyamide (LCAHA)
- the interaction of human single-stranded DNA binding protein 1 (hSSB1) with bloom syndrome protein helicase (BLM helicase)
- calcium-mediated actin reset (CaAR) in response to physiological changes
DNA Profile
STR-PCR Data: Amelogenin: X
CSF1PO: 13
D13S317: 13
D16S539: 11,12
D5S818: 11
D7S820: 11,12
THO1: 6,9.3
TPOX: 11,12
vWA: 14,18
Culture Medium
McCoy′s 5a medium with 1.5 mM Glutamine; 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split subconfluent 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh medium, aspirate and dispense into new culture flasks.
Other Notes
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biological source: human bone. growth mode: Adherent. karyotype: Not specified. morphology: Not specified. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. relevant disease(s): cancer. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 42296704
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 92022711-1VL
