U2A

General description

For complete product information, please see ECACC

Cell Line Description

The human sarcoma cell line HT 1080 (ECACC Catalogue number 85111505) was transfected with a vector encoding a selectable marker (guanine phosphoribosyltransferase) regulated by alpha interferon to create the 2fTGH cell line, enabling the selection of mutations in genes encoding components of the interferon signalling pathway. Chemical mutagenesis of the parent 2fTGH cell line enabled the isolation of a number of IFNg signalling mutants available from ECACC: U2A (12021502), U3A (12021503), U4A (12021504), U4C (12021505), U5A (12021506), U6A (12021507). Each contains a different mutation in the IFN signalling pathway. The 2fTGH panel provides a tool for the in vitro study and comparison of disrupted interferon signalling at multiple points across the IFN pathway. The U2A mutant cell line lacks expression of p48 gene product. U2A is unresponsive to interferon-alpha and interferon-beta; furthermore it is partially defective in response to interferon-gamma.

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:8 i.e. seeding at 1-4x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO2; 37°C.

Other Notes

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Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.