This enzyme is responsible for the first step in ubiquitin-protein isopeptide bond formation, by forming a high-energy thioester bond with ubiquitin. The ubiquitin is activated by first adenylating with ATP its Cterminal glycine residue (Gly76) and thereafter linking this residue to the side chains of a cysteine residue in UBE1, yielding an ubiquitin-UBE1 thiolester and free AMP and PPi. The activated ubiquitin is then transferred to a lysine of the targeted protein via the E2-E3 conjugation cascade. UBE1 is a critical component for the initiation of any in vitro conjugation reactions. We recommend an initial Recombinant Human Ubiquitin Activating Enzyme (UBE1) concentration of 50-200 nM. Specification: 0.5 μg/μl in PBS containing 0.01% sodium azide EC Number: 230-907-9 Related Documents: https://ald-pub-files.oss-cn-shanghai.aliyuncs.com/aladdinsci/pdp/sds/1/U388907-SCI_7802e6a1e63a36d56c3f42b67c1b8ee2.pdf
- UPC:
- 12352200
- Condition:
- New
- Availability:
- 8-12 weeks
- Weight:
- 1.23 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- U388907-50μl
- Product Size:
- 50μl
- Precautionary Statement Codes:
- P264
