UltraBioâ„¢ RNA Clean Magnetic Beads (C007B-566001)

Aladdin's UltraBio™ RNA Clean Magnetic Beads are coated with a novel nucleic acid purification resin by the solid phase reverse immobilization (SPRI) technology. The buffer system of this product are optimized to allow for stable, efficient and convenient purification of RNA or cDNA from common enzyme reactions. The product is suitable not only for manual operations, but also for high-throughput automated workstations.RNA purification are essential for RNA-related experiments, such as RNA-seq, qPCR, and microarray [1]. This product can be widely used in gene sequencing and molecular diagnostics, especially for RNA purification after rRNA removal or DNase I treatment, in vitro transcript purification and RNA purification during next generation sequencing (NGS) library preparation [2]. This product is basically the same as the widely used RNAClean XP (Beckman Coulter) in terms of usage and effectiveness.This product can be used to purify RNA, cDNA or double-stranded DNA. This product cannot differentiate between RNA or DNA, but samples can be treated with DNase before RNA purification or with RNase before DNA purification. This product is not suitable for direct RNA extraction from cells or tissues.Please refer to Figure 1 for the workflow of this product. RNA in samples binds to UltraBio™ RNA Clean Magnetic Beads under appropriate conditions. With a magnetic stand, the magnetic beads can be separated from the solution rapidly and efficiently. After removing the supernatant followed by several times of bead washing, the high-purity RNA can be eluted from the magnetic beads with the Elution Buffer.Figure 1. The workflow of Aladdin's UltraBio™ RNA Clean Magnetic Beads .This product is easy to operate and enables RNA purification to be completed within 15 minutes. The product can recover over 90% of RNA and is also suitable for the purification of RNA at low concentrations.Compared with the traditional RNA recovery method, this product avoids the use of organic solvents such as phenol and chloroform, which is safer and more eco-friendly.With strong specificity and high RNA binding capacity, this product can rapidly purify RNA from different systems and effectively remove unincorporated dNTP or NTP, primers, primer dimers, salts and other impurities. Please refer to Figure 2 for the performance of this product in purifying in vitro transcribed RNA of different lengths.Figure 2. Purification of RNA of different length using the UltraBio™ RNA Clean Magnetic Beads . Four linearized plasmids carrying T7 Promoter sequences were transcribed into RNA of different lengths by T7 Quick High Yield RNA Transcription Kit in vitro, respectively. The transcription products were digested by RNase-free DNase I to remove the DNA template, followed by purification with this product. As shown in the figure, the recovery efficiency of RNA was very high. This figure is for reference only, which may vary due to different experimental conditions.Precautions：Absolute ethanol and magnetic separation racks are required but not supplied in this kit. We recommend 1.5/2ml Magnetic Separation Rack (FMS004, FMS008, FMS012, FMS016, FMS024).Strict measures must be taken to prevent RNase contamination during the operation process. Avoid exhaling or speaking and wear a disposable mask during the operation. For removal of RNase in the operation environment, it is recommended to use RNase and DNase Away (, R0123) to remove RNase from the laboratory bench, instrument surface, or other contact surfaces.All home-made reagents and consumables should be RNase-free. If the consumables may be contaminated with RNase, soak them in 0.01% DEPC water overnight, then autoclave and dry. Do not freeze or centrifuge this product at high speed.To ensure the recovery efficiency of RNA, the magnetic beads should be equilibrated to room temperature before use.The magnetic beads must be resuspended thoroughly before use by vortex or inversion of the tube.We recommend preparing the 80% (v/v) ethanol solution freshly. Otherwise the recovery efficiency may decrease due to the volatilization of ethanol.Do not dry the beads for a long time. Excessive drying will lead to irreversible aggregation of the beads and thus the reduction of elution efficiency.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.Instructions for Use：1. Preparation of magnetic beadsa. Take out the magnetic bead solution at 4℃, vortex or invert the tube upside down properly to resuspend the beads thoroughly. Take the required amount of beads and equilibrate to room temperature.b. Prepare 80% (v/v) ethanol solution freshly. For example, mix 8 ml of absolute ethanol and 2 ml of RNase-free water to obtain approximately 10 ml of 80% (v/v) ethanol. The Ultrapure Water (DNase/RNase-Free, Sterile) ( RNA Clean Magnetic Beads (well mixed and equilibrated to room temperature) according to the table below. Mix by gentle vortex or pipetting up and down 10 times and incubate at room temperature for 5 minutes. Then place the centrifuge tube in a magnetic rack and incubate at room temperature for 30 seconds. After the solution is clear, carefully aspirate and discard the supernatant.Sample Volume (µl) Beads (µl) 50100100200150300200400Note 1: Magnetic beads must be mixed thoroughly prior to use. If necessary, increase the amount of magnetic beads or extend the binding time to improve the yield.Note 2: In general, the amount of magnetic beads is 1.8-2.0 times the sample volume. The amount of magnetic beads can also be adjusted appropriately based on the actual test results.c. Keep the centrifuge tube in the magnetic rack, add 200µl of freshly prepared 80% (v/v) ethanol without disturbing the beads, and incubate at room temperature for 30 seconds. Carefully remove the supernatant.d. Repeat step c once.e. Keep the centrifuge tube in the magnetic rack, air dry the beads with the lid open at room temperature until cracks just appear (5-10 minutes).Note: Do not over-dry the beads that may result in lower elution efficiency.f. Remove the centrifuge tube from the magnetic rack. Add an appropriate amount of ddH2O (10-30µl) and mix thoroughly by vortex or pipetting.g. Incubate at room temperature for 5 minutes.h. Spin the tube briefly and place it on a magnetic rack for 5 minute until the solution is clear. Carefully aspirate the supernatant into a clean microcentrifuge tube for downstream applications.Note: It is recommended to reserve a small amount of liquid when transferring the supernatant to avoid suctioning the magnetic beads that may affect subsequent experiments. As the purified RNA is highly susceptible to degradation, the downstream experiment should be performed immediately. Otherwise, keep at -80℃ for later use.