Aladdin's Uracil-DNA Glycosylase (Heat-labile, Cod), also known as Heat-labile Cod UDG, is a thermolabile UDG derived from Atlantic cod (Gadus morhua) and catalyzes the hydrolysis of the N-glycosidic bond between the uracil (dU) base and the deoxyribose moiety in uracil-containing DNA strands, thereby releasing free uracil. It works on single- or double-stranded DNA containing dU, but not RNA or DNA oligomers (6 or fewer bases).UDG is mainly used to eliminate carryover contaminations in PCR as followsApplication：Removal of uracil bases in single-stranded or double-stranded DNA; removal of cross contamination of PCR products; detection of DNA repair and mutagenesis; improve the cloning efficiency of PCR products; improve the mutagenesis efficiency; protein-DNA interaction studies. This product can be used in PCR, qPCR, RT-PCR, RT-qPCR, and one-step RT-PCR, etc.Enzyme storage buffer: 50mM Tris-HCl (pH 7.5), 100mM NaCl, 0.5mM EDTA, 1mM DTT, 0.1% Triton X-100, 50% (v/v) glycerol.10X Heat-labile Cod UDG Buffer: 700mM Tris-HCl, 100mM NaCl, 10mM EDTA, 1mg/ml BSA, pH 8.0 at 25℃.Inactivation or inhibition: Heating at 50℃ for 10 min can completely inactivate the Heat-labile Cod UDG.Source：Recombinant Uracil-DNA Glycosylase expressed and purified from E. coli.Purity: Free of DNA endonuclease and exonuclease, phosphatase, and RNase.Applications: Removal of uracil bases in single-stranded or double-stranded DNA; removal of cross contamination of PCR products; detection of DNA repair and mutagenesis; improve the cloning efficiency of PCR products; improve the mutagenesis efficiency; protein-DNA interaction studies. This product can be used in PCR, qPCR, RT-PCR, RT-qPCR, and one-step RT-PCR, etc.Enzyme storage buffer：50mM Tris-HCl (pH 7.5), 100mM NaCl, 0.5mM EDTA, 1mM DTT, 0.1% Triton X-100, 50% (v/v) glycerol.Inactivation or inhibition：Heating at 50℃ for 10 min can completely inactivate the Heat-labile Cod UDG.Precautions：Heat-labile Cod UDG enzyme is active in most PCR reaction buffers, but it is recommended to examine its compatibility with a particular PCR reaction system used for the first time.The dNTP/dUTP is required but not supplied. We recommend using dNTP/dUTP Mixture (2.5mM each/5mM) .Heat-labile Cod UDG should be stored at -80℃ for long-term storage and -20℃ for routine use. Avoid repeated freeze-thaws.When using the Heat-labile Cod UDG enzyme for One-Step RT-PCR or One-Step qRT-PCR, a heat-resistant reverse transcriptase should be utilized and the reverse transcription should be performed at 50-55℃.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.Instructions for Use：1. Set up the PCR or RT-PCR reactions as usual, add Heat-labile Cod UDG to the final concentration of 0.01U/μl, and mix well. Usually, it is not necessary to add the UDG buffer.2. Incubate at 25℃ for 5 min, and then proceed to PCR or RT-PCR cycling conditions.
Specification: Free of DNA endonuclease and exonuclease, phosphatase, and RNase.
- UPC:
- 12352204
- Condition:
- New
- Availability:
- 4-8 weeks
- Weight:
- 0.07 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- U751666-5000U
- Product Size:
- 5000U
