Urea Agar Base Media is a slight modification of Christensen formulation and is recommended by BIS for identification of urease activity. Rustigian and Stuart had originally formulated a medium to detect urease activity. These media differentiate between rapid urease positive Proteus species and other urease positive organisms like Citrobacter , Enterobacter and Klebsiella and the bacteria other than Enterobacteriaceae . Christensen observed that addition of peptic digest of animal tissue, dextrose and reduced content of buffer helps to support an early luxuriant growth. Heavy inoculum of growth is inoculated on the surface of the slants. When urea is utilized, ammonia is formed during incubation which makes the medium alkaline, showing a pink-red color by the change in the phenol red indicator. Prolonged incubation may cause alkaline reaction in the medium. Check using medium without urea as the negative control. Storage and Shelf-life: Store below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label.
- UPC:
- 41106200
- Condition:
- New
- Availability:
- 3 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- M112S-500G
