Cell Line Origin
Human skin epithelioid sarcoma
Cell Line Description
Spontaneously transformed cells from a male patient with epithelioid sarcoma derived from bone marrow metastasis. Cells grow very rapidly and secrete GM-CSF. They have been shown to express AE-1 and AE-3 cytokeratins, epithelial membrane antigen, p53, human Fas and vimentin. VA-ES-BJ cells are sensitive to oxidising agents, busulfaxamine and protoporphyrin IX. Epithelial, spindle and giant cells.
Application
Study of tumours. Tumourigenic in nude mice.
Culture Medium
DMEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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biological source: human skin (bone marrow metastasis). growth mode: Adherent. karyotype: 66-68 Aneuploidic, pentasomy #7, multiple trisomy. morphology: Epithelial. products: Granulocyte Macrophage Colony Stimulating Factor (GM-CSF). receptors: Granulocyte Macrophage Colony Stimulating Factor (GM-CSF), AE, AE3, Fas, epithelial membrane antigen (EMA), Vimentin, P53. technique(s): cell culture | mammalian: suitable. relevant disease(s): metastasis. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 51202301
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 94092303-1VL