Vascular Endothelial Growth Factor 165; Human VEGF165, 10ug

VEGF165, Vascular Endothelial Growth Factor 165, human: Human VEGF 165 VEGF is a potent growth and angiogenic cytokine. It stimulates proliferation and survival of endothelial cells and promotes angiogenesis and vascular permeability. Expressed in vascularized tissues, VEGF plays a prominent role in normal and pathological angiogenesis. Substantial evidence implicated VEGF in the induction of tumor metastasis and intra-ocular neovascular syndromes. VEGF signals through three receptors; fms-like tyrosine kinase (flt-1), KDR gene product (the murine homolog of KDR is flk-1 gene product) and the flt4 gene product. Recombinant Human VEGF165 (rHuVEGF165) Source: Escherichia coli. Molecular Weight: Recombinant human VEGF165 is a 38.2 kDa disulfide-linked homodimeric protein consisting of two 165 amino acid polypeptide chains. Purity: >95% by SDS-PAGE and HPLC analyses. Biological Activity: Fully biologically active when compared to standard. Determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 1.0-8.0 ng/ml.  Physical Appearance Sterile Filtered White Lyophilized (freeze-dried) powder Endotoxin: Less than 1EU/mg of rHuVEGF165 as determined by LAL method. Formulation: Lyophilized from a 0.2mm filtered concentrated solution in PBS, pH 7.4. Amino Acid Sequence: APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQETP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQHQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR