Viral Nucleic Acid Extraction Kit - 100 Preps

Sample: 200 μl sample (plasma, serum, body fluid or the sup ernatant of viral infected cell cultures) Format: Spin column Rxns: 50,100 and 300 Operation time: 40 minutes IBI’s Viral Nucleic Acid Extraction Kit was designed for purification of viral DNA/RNA from cell-free samples; such as serum, plasma, body fluids, and the supernatant of viral infected cell cultures. DNA/RNA viruses are lysed quickly and efficiently using the lysis buffer, which is a highly concentrated solution of chaotropic salt. When combined with ethanol, the AD Buffer creates an optimum condition for nucleic acid binding to the glass fiber matrix of the column. Contaminants such as salts, metabolites, and soluble macromolecular cellular components are removed in the wash process. Nucleic Acid is eluted in RNase-free water and is then ready for use in subsequent reactions, including Real-Time PCR, Automated Fluorescent DNA Sequencing, PCR, and other enzymatic reactions. The detection limit for certain viruses depends on the sensitivity of the individual PCR or RT-PCR assays.