VivoTrack 680 NIR Fluorescent Imaging Agent

VivoTrack 680 is a water soluble NIR (near infrared) fluorescent cell labeling agent that can label a variety of cell types. As cell migration is critical for many important in vivo biological functions, this labeling approach has the potential to provide a useful tool for research in inflammation, immunology and stem cells.  VivoTrack 680 can label cells brightly and uniformly without requiring harsh organics such as DMSO or ethanol, chemicals known to affect cell function. The labeled cells retain excellent viability and normal proliferative capacity, and significant signal is retained in dividing cells in vitro for more than 7 days. Many different cell types can be labeled for in vivo transfer or implantation, and in vivo utility is illustrated by non-invasively tracking fluorescent primary macrophages to sites of inflammation by NIR fluorescent imaging.Features/Benefits1. Track stem cells, T cells, macrophages and many other cell types longitudinally in vivo 2. Water soluble - no need for harsh organics such as DMSO or ethanol, chemicals known to affect cell function Homogeneous cell staining with no impact on cell viability. 3.Optimized for in vivo imaging instrumentProcedure1. Wash the cells of interest once with sterile PBS or serum-free medium to remove serum proteins and lipids that may interfere with cell labeling. 2. Discard the supernatant and resuspend the cells (up to 250 x 106 cells/mL) in 2.0 mL of PBS in a 50 mL sterile conical tube. 3. Dissolve VivoTrack 680 (0.2mg of dye in 1g of PEG5000) in 1.3 mL of warm sterile PBS (37 °C) and mix by vortex until completely dissolved. This will yield 2.0 mL of the labeling agent. 4. Add 2.0 mL of the cell labeling solution to 2.0 mL of cells, and mix immediately by gentle vortexing. [Note: It may be required to optimize dose depending on the cells to be labeled.] 5. Incubate the cells for 15 min at room temperature, protected from light. 6. Dilute the cells for washing by adding 15-20 mL sterile RT PBS containing 1% FBS or complete medium, depending on your ultimate use for the cells. 7. Wash the cells 3 times with RT PBS containing 1% FBS to remove excess cell labeling agent. A final resuspension with sterile PBS alone can be used to decrease the FBS levels in the cell preparation. 8. Count, culture, or transfer cells as required by the application. VivoTrack 680 is highly soluble in aqueous solution and, thus, does not need to transition from organic to aqueous solution upon addition to the cell suspension. This allows quick dispersion into the cell medium, and very uniform cell labeling is generally achieved. For laboratory use only. This product is intended for animal research only and not for use in humans. Specifications and Purity: 90%. Related Document: https://ald-pub-files.oss-cn-shanghai.aliyuncs.com/aladdinsci/pdp/sds/1/V278914-SCI_c7a8fa12d7bc7016af30b80ed33fecd2.pdf.