Cell Line Origin
Mouse NS0 x Mouse BALB/c spleen
Cell Line Description
The antibody cross-reacts with bovine and human MHC class II. It reacts with MHC class II molecules on cell surfaces and on immunoblots. It reacts very weakly with ovine DQ L cell transfectants by immunofluorescence, but does not react with DQ blots and therefore is classified as strongly DR α specific, but weakly cross-reactive with DQ α.
Immunogen
Ovine MHC class II
Application
Quantification of DQ and DR expression on T cell subsets in adult and foetal peripheral blood, afferent lymph, lymph node and efferent lymph.
Culture Medium
RPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS)
Subculture Routine
Maintain cultures between 1-2x100,000 cells/ml; 5% CO2; 37°C. Saturation density is reached at 3-9x100,000 cells/ml.
Other Notes
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biological source: Mouse x Mouse Hybridoma. ImmunoDon: BALB/c mouse spleen. Myeloma: NS0. Reactivity: Anti-MHC class II α. -chain, ovine. technique(s): cell culture | mammalian: suitable. isotype: IgG1. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 41106513
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 93113029-1VL