Cell Line Origin
Mouse NS0 x Mouse BALB/c spleen
Cell Line Description
The antibody cross-reacts with bovine MHC class II molecules and reacts with MHC class II molecules on cell surface and on immunoblots. It reacts with the majority, but not all DQß chains.
Immunogen
Ovine MHC class II
Application
Quantification of DR expression on T cell subsets in adult and foetal peripheral blood, afferent lymph, lymph node and efferent lymph.
Culture Medium
RPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS)
Subculture Routine
Maintain cultures between 1-2x100,000 cells/ml; 5% CO2; 37°C. Saturation density reached at 3-9x100,000 cells/ml. (takes 3-4 days to reach 5x100,000)
Other Notes
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biological source: Mouse x Mouse Hybridoma. ImmunoDon: BALB/c mouse spleen. Myeloma: NS0. Reactivity: Anti-MHC class II DQβ. chain, ovine. technique(s): cell culture | mammalian: suitable. isotype: IgG1. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 51343305
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 93113031-1VL
