Cell Line Origin
Mouse BALB/c fibrosarcoma
Cell Line Description
Established from a fibrosarcoma induced by subcutaneous injection of methylcholanthrene in BALB/c mice. Highly sensitive after pre-treatment with actinomycin D to human cytotoxic monocytes, to tumour necrosis factor and to lymphotoxin. It provides convenient target cells for short-term (51) chromium release assays.
Application
Tumourigenicity and cytotoxicity studies, chromium release assays
Culture Medium
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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biological source: mouse skin (fibroblast). growth mode: Adherent. karyotype: Not specified. morphology: Fibroblast. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. relevant disease(s): cancer. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 41106514
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 87022501-1VL
