Western Blot Blocker, 500g

Western Blot Blocker (Thomas No. C994D14 and C99 D15) For those that prefer animal protein as the blocking agent for your immuno-detection analysis. Modified milk protein that is highly soluble and does not inhibit peroxidase detection. The modified milk protein has high blocking efficiency with a clear background. Features: Readily soluble and produces semi-clear solution No inhibition to peroxidase Produces clear background Higher blocking efficiency Blocking time 30-60 minutes Fat free Re-Probe Solution, Western Blots (Thomas No. C994D16 ) For those that prefer animal protein as the blocking agent for your immuno-detection analysis. Modified milk protein that is highly soluble and does not inhibit peroxidase detection. The modified milk protein has high blocking efficiency with a clear background. Features: Readily soluble and produces semi-clear solution. No inhibition to peroxidase. Produces clear background. Higher blocking efficiency Blocking time 30-60 minutes Fat free Re-Probe Solution, Western Blots (Thomas No. C994D17) Re-Probe is supplied as 5X The 125ml kit sufficient for 25 blots Not recommended for chromogenic substrates Multiple Species Blot of Brain Tissue (Thomas No. C994D18) Tissue specific total protein extracted from rat, human, and mouse Blots are prepared by extracting total tissue specific proteins in a denaturing buffer containing a cocktail of protease inhibitors to minimize proteolytic damage to proteins. 50 µg of protein from each sample is loaded along side colored marker proteins on 4-20% denaturing polyacrylamide gels and electrophoresed. After electrophoresis, the gel is electro-blotted onto PVDF membranes. These blots are ready to be probed with polyclonal, monoclonal, or peptide antibodies. Specifications: Components (Multiple Species Tissue Blot): Preblotted Strip of Proteins Instruction Manual Storage Condition and Stability: Stable for 6 months at 4µC Multiple Tissue Blot (Human) Multiple tissue blots are prepared using total protein extracted from a variety of tissue types, selected from a single species Tissues included: Liver Lung Brain Kidney Testis Ovary Heart Spleen Pancreas Blots are prepared by extracting total tissue specific proteins in a denaturing buffer containing a cocktail of protease inhibitors to minimize proteolytic damage to proteins. 50 μg of protein from each sample is loaded along side colored marker proteins on 4-20% denaturing polyacrylamide gels and electrophoresed. After electrophoresis, the gel is electro-blotted onto PVDF membranes. These blots are ready to be probed with polyclonal, monoclonal, or peptide antibodies. Components (Multiple Tissue Blot): Preblotted Membrane of Proteins Instruction Manual Storage Conditions and Stability: Stable for 6 months at 4°C