Western ice free bath rapid film transfer solution (10 ×） (C09-1185-065)

Product introduction：Western ice free bath rapid film transfer solution (10 ×） It is a kind of wet transfer membrane solution with stronger buffer capacity, more thorough protein transfer membrane, no ice bath and shorter transfer membrane time. This product is safe and non-toxic. Generally, the film transfer process can be completed in 30 min and diluted into 1 × It can be reused for 2-3 times. Limiting dilution to 1 × After that, 1000 ml of film transfer liquid is used for electro rotation each time, and the product can be used for 15 times when it is reused for 3 times.Matters needing attention：1. this product is only for scientific research. 2. for your safety and health, please wear experimental clothes and disposable gloves.Scope of application：Western membrane transfer solution Instruction： 1.Making method 1000 mL rapid transfer buffer ( 1 × ) : 100 mL Western ice-free rapid transfer solution ( 10 × ) + 700 mL deionized water + 200 mL methanol. 2.Transmembrane conditions The constant current was set to 400 mA, and the < 150 kDa protein could be completely transferred for 30 min. Note : ①PVDF membrane needs to be soaked with anhydrous methanol for more than 30 s before use.② The transfer time of different gel concentration and different transfer protein size needs to be adjusted.