Western ReProbe [5X], 1 Gallon

Western ReProbe™ is a single component system that is specifically formulated to dissociate and remove antibodies from membrane-bound proteins without destroying the antigenic binding affinity. Western ReProbe™ also enables the ability to reuse western blots. Western ReProbe™ breaks the antigen-antibody binding affinity without using denaturants, SDS or boiling. The membrane-bound protein is retained on the membrane, and the matching antibodies are washed away. Once the antigen-antibody bonds are broken, the membrane-bound protein is free to accept new proteins. Removal of antibodies also removes corresponding chemiluminescent or radio-isotopic signals from the blots. The stripped blots can then be probed with new probes (see figure 1). Western ReProbe™ is not recommended for stripping color-producing western blots that use substrates such as TMB, chloronapthol and DAB. Western ReProbe™ is supplied as a 5X concentrated solution. Each 100 milliliters is sufficient for 25 to 30 stand ard size (7.5cm x 8.5cm) western blots. For high affinity antibodies, we recommend our Western ReProbe™ PLUS. Features Simply incubate the blot in Western ReProbe™ at room temp & wash No boiling, denaturants or SDS required Repeated analysis of Western blots with multiple probes Re-analysis & correction of unsatisfactory Western blots Conservation of hard-to-obtain test samples & reagents Time saving in not rerunning gels & transfer processes Applications Screen same blot with different antibodies Reprobe for housekeeping proteins Compare phosphorylated & total protein on the same blot